LOCUS New_DNA 12835 bp ds-DNA circular 07-FEB-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from 92036 pORF-CDKN2D_ORS04524 652 to 1185 COMMENT Backbone from 90946 PB-tet-PH-lox66FRT2 6253 to 4570 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 5094..5104 /mol_type="other DNA" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /db_xref="taxon:32630" /organism="synthetic construct" /clone_lib="HIP_Gateway221_closed /focus /note=Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1205..1459 /label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 source 4581..4592 /mol_type="other DNA" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /db_xref="taxon:32630" /organism="synthetic construct" /clone_lib="HIP_Gateway221_closed /focus /note=Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE:(1) /ApEinfo_label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3331..3678 /label=polyA /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(463..531) /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5094..5094 /note="linker at the 3' end of the ORF" /label=linker at the 3' end of the ORF /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1460..1711 /label=mouse PGK promoter(1) /ApEinfo_label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 707..941 /label=PB 3'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4593..5093 /label=CDKN2D(BC001822) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 5828..5896 /label=LacZ alpha(1) /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4446..4551 /label=mCMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4581..4587 /note="attL1 site" /label=attL1 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5151..5674 /label=IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(10458..10491) /label=CoreFRT /ApEinfo_fwdcolor=#8080c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3685..3926 /label=chicken 5'-HS4 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5095..5104 /note="attL2 site" /label=attL2 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4588..4592 /note="linker at the 5' end of the ORF that includes ACC for minimal Kozak consensus" /label=linker at the 5' end of the ORF that includes ACC /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10378..10411 /label=lox66 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4160..4437 /label=8xTetO2 element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(5105..5119) /label=AttR2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 602..619 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10444..10491 /label=FRT /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 618..645 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5676..9618 /label=b-geo /ApEinfo_fwdcolor=#800000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1732..2328 /label=Pac /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5739..8764 /label=LacZ /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 9619..9925 /label=pA /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10444..10456 /label=FRTunit /ApEinfo_fwdcolor=#80ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10043..10313 /label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(10642..10661) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(135..441) /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(10679..10699) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(3..458) /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(11067..11749) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2335..3330 /label=DeltaTK /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding complement(10705..10727) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(11847..12506) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4563..4570 /label=AttR1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10314..10355 /label=PB 5'LTR(1) /ApEinfo_label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG 661 TATTCACGAC AGCAGGCTGA ATAATAAAAA AATTAGAAAC TATTATttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaAA CATTCTCTCT 961 TTTACAAAAA TAAACTTATT TTGTACTTTA AAAACAGTCA TGTTGTATTA TAAAATAAGT 1021 AATTAGCTTA ACCTATACAT AATAGAAACA AATTATACTT ATTAGTCAGT CAGAAACAAC 1081 TTTGGCACAT ATCAATATTA TGCTCTCGTT ACTGGCGAAA GGGGGATGTG CTGCAAGGCG 1141 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA 1201 ATTCtaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 1261 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 1321 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 1381 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 1441 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 1501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 1561 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 1621 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 1681 ctcctcttcc tcatctccgg gcctttcgac cGATCATCAA GCTTGATCCT Catgaccgag 1741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccAgggccgt acgcaccctc 1801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgaTccgga ccgccacatc 1861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 1921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 1981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 2041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 2101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 2161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 2221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 2281 Gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccATGCCC 2341 ACGCTACTGC GGGTTTATAT AGACGGTCCC CACGGGATGG GGAAAACCAC CACCACGCAA 2401 CTGCTGGTGG CCCTGGGTTC GCGCGACGAT ATCGTCTACG TACCCGAGCC GATGACTTAC 2461 TGGCGGGTGc TGGGGGCTTC CGAGACAATC GCGAACATCT ACACCACACA ACACCGCCTC 2521 GACCAGGGTG AGATATCGGC CGGGGACGCG GCGGTGGTAA TGACAAGCGC CCAGATAACA 2581 ATGGGCATGC CTTATGCCGT GACCGACGCC GTTCTGGCTC CTCATATCGG GGGGGAGGCT 2641 GGGAGCTCAC ATGCCCCGCC CCCGGCCCTC ACCCTCATCT TCGACCGCCA TCCCATCGCC 2701 GCCCTCCTGT GCTACCCGGC CGCGCGgTAC CTTATGGGCA GCATGACCCC CCAGGCCGTG 2761 CTGGCGTTCG TGGCCCTCAT CCCGCCGACC TTGCCCGGCA CcAACATCGT GcTTGGGGCC 2821 CTTCCGGAGG ACAGACACAT CGACCGCCTG GCCAAACGCC AGCGCCCCGG CGAGCGGCTg 2881 GACCTGGCTA TGCTGGCtGC GATTCGCCGC GTTTAcGGGC TaCTTGCCAA TACGGTGCGG 2941 TATCTGCAGt GCGGCGGGTC GTGGCGGGAG GAcTGGGGAC AGCTTTCGGG GaCGGCCGTG 3001 CCGCCCCAGG GTGCCGAGCC CCAGAGCAAC GCGGGCCCAC GACCCCATAT CGGGGACACG 3061 TTATTTACCC TGTTTCGGGC CCCCGAGTTG CTGGCCCCCA ACGGCGACCT GTATAACGTG 3121 TTTGCCTGGG CcTTGGACGT CTTGGCCAAA CGCCTCCGTT CCATGCACGT CTTTATCCTG 3181 GATTACGACC AATCGCCCGC CGGCTGCCGG GACGCCCTGC TGCAACTTAC CTCCGGGATG 3241 GTCCAGACCC ACGTCACCAC CCCCGGCTCC ATACCGACGA TATGCGACCT GGCGCGCACG 3301 TTTGCCCGGG AGATGGGGGA GGCTAACTGA gctctagctt atcgactcgc cgatagtgga 3361 aaccgacgcc ccagcactcg tccgagggca aaggaatagt cgagaaattg atgatctatt 3421 aaacaataaa gatgtccaca tggaagtttt tcctgtcata ctttgttaag aagggtgaga 3481 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga 3541 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca 3601 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca 3661 ctcatgatct atagatccTc tagaGAGCTC ACGGGGACAG CCCCCCCCCA AAGCCCCCAG 3721 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 3781 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 3841 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 3901 CACCTGGGGG ATACGGGGAA AAAGCTgtcg aCCTGCAGGC ATGCAAGCTT GGCGTAATCA 3961 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4021 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4081 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGTA ACTCGAGGGA 4141 TCCAAGCTgg CCgcACGTCT CCCTATCAGT GATAGAGAAG TCGACACGTC TCGAGCTCCC 4201 TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT CAGTGATAGA GAAGTCGACA 4261 CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA GAACGTCTCC CTATCAGTGA 4321 TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG AGAAGGTACG TCTAGAACGT 4381 CTCCCTATCA GTGATAGAGA AGTCGACACG TCTCGAGCTC CCTATCAGTG ATAGAGAAGG 4441 TACCCCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC 4501 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GTGGCGGCCA 4561 TCACAAGTTT gtacaaaaaa gcaggctcca ccatgctgct ggaggaggtt cgcgccggcg 4621 accggctgag tggggcggcg gcccggggcg acgtgcagga ggtgcgccgc cttctgcacc 4681 gcgagctggt gcatcccgac gccctcaacc gcttcggcaa gacggcgctg caggtcatga 4741 tgtttggcag caccgccatc gccctggagc tgctgaagca aggtgccagc cccaatgtcc 4801 aggacacctc cggtaccagt ccagtccatg acgcagcccg cactggattc ctggacaccc 4861 tgaaggtcct agtggagcac ggggctgatg tcaacgtgcc tgatggcacc ggggcacttc 4921 caatccatct ggcagttcaa gagggtcaca ctgctgtggt cagctttctg gcagctgaat 4981 ctgatctcca tcgcagggac gccaggggtc tcacaccctt ggagctggca ctgcagagag 5041 gggctcagga cctcgtggac atcctgcagg gccacatggt ggccccgctg taggacccag 5101 ctttCTTGTA CAAAGTGGTG ATggccGCTC TAGCTAGTCG ATAAGCTAAT TCCGCCCCCC 5161 CCCCCCCCCC CCCCTCACCc TCCCCCCCCC ctaacgttac tggccgaagc cgcttggaat 5221 aaggccggtg agcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg 5281 tgagggcccg aaacctggcc ctgtcttctt gacgacgatt cctaggggtc tttcccctct 5341 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 5401 ttgaagacaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 5461 atggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa 5521 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt 5581 tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 5641 ttggtttgaa aaacacgatg ataatatggc cacaaccatg gaacaggggg atcagcttac 5701 catggccaag atccctccta agaagaagcg caaagtcgag gatcccgtcg ttttacaacg 5761 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 5821 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 5881 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg 5941 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca 6001 cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt 6061 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg 6121 gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg 6181 gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct 6241 gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga 6301 cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc 6361 gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga 6421 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta 6481 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc 6541 tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct 6601 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt 6661 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg 6721 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg 6781 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt 6841 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc 6901 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc 6961 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct 7021 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt 7081 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg 7141 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac 7201 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc 7261 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct 7321 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg 7381 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca 7441 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg 7501 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc 7561 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg 7621 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca 7681 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc 7741 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca 7801 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca 7861 catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc 7921 gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa 7981 taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa 8041 aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat 8101 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc 8161 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc 8221 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg 8281 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc 8341 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga 8401 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc 8461 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag 8521 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg 8581 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg 8641 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat 8701 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg 8761 ctaccattac cagttggtct ggtgtcaggg gatcccccgg gctgcagcca atatgggatc 8821 ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 8881 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 8941 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 9001 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 9061 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 9121 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 9181 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 9241 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 9301 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 9361 cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 9421 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 9481 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 9541 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 9601 tgacgagttc ttctgagggg atcaattctc tagggctcgc tgatcagcct cgactgtgcc 9661 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 9721 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 9781 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 9841 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 9901 ctggggctcg atcctctaga gtcgaGGGCT GCAGGAATTC GATATCAAGC TCTAGCCAAC 9961 AAGCTCGTCA TCGCTTTGCA GAAGAGCAGA GAGGATATGC TCATCGTCTA AAGAACTACC 10021 CATTTTATTA TATATTAGTC ACgatatcta taacaagaaa atatatatat aataagttat 10081 cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca 10141 cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga 10201 cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta 10261 aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca agaATGCATg 10321 cgtcaatttt acgcagacta tctttctagg gttaaAtttG GGCGGTCGAG GGACCTAata 10381 acttcgtata GCATACAtta tacgaacggt aATTAAGGGT TCCGGATCCC CATCAAGCTA 10441 ATTgaagttc ctattcCgaa gttcctattc TCTAGAAAgt ataggaactt cCAGCTGGAA 10501 AtGATTTGCG CTTTACTCGA CCTAAACTTT AAACACGTCA TAGAATCTTC GTTTGACAAA 10561 AACCACATTG TGGCCAAGCT GTGTGACGCG ACGCGCGCTA AAGAATGGCA AACCAAGTCG 10621 CGCGAGGTAC CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTCCGAGCTT GGCGTAATCA 10681 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 10741 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 10801 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 10861 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 10921 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 10981 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 11041 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 11101 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 11161 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 11221 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 11281 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 11341 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 11401 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 11461 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 11521 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 11581 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 11641 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 11701 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 11761 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 11821 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 11881 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 11941 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 12001 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 12061 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 12121 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 12181 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 12241 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 12301 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 12361 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 12421 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 12481 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 12541 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 12601 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 12661 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 12721 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 12781 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTG //