LOCUS New_DNA 13428 bp ds-DNA circular 21-APR-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from 92239 pORF-ISL2 652 to 1778 COMMENT Backbone from 90946 PB-tet-PH-lox66FRT2 6253 to 4570 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 5575..5679 /label=sequenced /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1205..1459 /label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 707..941 /label=PB 3'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4600..5079 /label=sequenced(1) /ApEinfo_label=sequenced /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3331..3678 /label=polyA /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 gene 4600..5679 /gene="ISL2" /db_xref="GeneID:64843" /db_xref="HGNC:18524" /db_xref="MIM:609481" /label=ISL2 /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#004080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1460..1711 /label=mouse PGK promoter(1) /ApEinfo_label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 4600..5679 /gene="ISL2" /codon_start=1 /product="ISL LIM homeobox 2" /protein_id="AAH12136.1" /db_xref="GI:15082435" /db_xref="GeneID:64843" /db_xref="HGNC:18524" /db_xref="MIM:609481" /translation="MVDIIFHYPFLGAMGDHSKKKPGTAMCVGCGSQIHDQFILRVSP DLEWHAACLKCAECSQYLDETCTCFVRDGKTYCKRDYVRLFGIKCAKCQVGFSSSDLV MRARDSVYHIECFRCSVCSRQLLPGDEFSLREHELLCRADHGLLLERAAAGSPRSPGP LPGARGLHLPDAGSGRQPALRPHVHKQTEKTTRVRTVLNEKQLHTLRTCYAANPRPDA LMKEQLVEMTGLSPRVIRVWFQNKRCKDKKKSILMKQLQQQQHSDKTSLQGLTGTPLV AGSPIRHENAVQGSAVEVQTYQPPWKALSEFALQSDLDQPAFQQLVSFSESGSLGNSS GSDVTSLSSQLPDTPNSMVPSPVET" /label=ISL LIM homeobox 2 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ffff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 6421..6489 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4160..4437 /label=8xTetO2 element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4600..4839 /label=sequenced(2) /ApEinfo_label=sequenced /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5744..6267 /label=IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3685..3926 /label=chicken 5'-HS4 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5412..5679 /label=sequenced(3) /ApEinfo_label=sequenced /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5386..5411 /label=sequenced(4) /ApEinfo_label=sequenced /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10971..11004 /label=lox66 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 602..619 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4600..5316 /label=sequenced(5) /ApEinfo_label=sequenced /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4446..4551 /label=mCMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4982..5679 /label=sequenced(6) /ApEinfo_label=sequenced /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(11051..11084) /label=CoreFRT /ApEinfo_fwdcolor=#8080c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 618..645 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(5698..5712) /label=AttR2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1732..2328 /label=Pac /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11037..11084 /label=FRT /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6269..10211 /label=b-geo /ApEinfo_fwdcolor=#800000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6332..9357 /label=LacZ /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10212..10518 /label=pA /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11037..11049 /label=FRTunit /ApEinfo_fwdcolor=#80ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(135..441) /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10636..10906 /label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(3..458) /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11235..11254) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2335..3330 /label=DeltaTK /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11272..11292) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(11660..12342) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4563..4570 /label=AttR1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding complement(11298..11320) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(12440..13099) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10907..10948 /label=PB 5'LTR(1) /ApEinfo_label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(463..531) /label=LacZ alpha(1) /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG 661 TATTCACGAC AGCAGGCTGA ATAATAAAAA AATTAGAAAC TATTATttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaAA CATTCTCTCT 961 TTTACAAAAA TAAACTTATT TTGTACTTTA AAAACAGTCA TGTTGTATTA TAAAATAAGT 1021 AATTAGCTTA ACCTATACAT AATAGAAACA AATTATACTT ATTAGTCAGT CAGAAACAAC 1081 TTTGGCACAT ATCAATATTA TGCTCTCGTT ACTGGCGAAA GGGGGATGTG CTGCAAGGCG 1141 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA 1201 ATTCtaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 1261 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 1321 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 1381 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 1441 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 1501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 1561 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 1621 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 1681 ctcctcttcc tcatctccgg gcctttcgac cGATCATCAA GCTTGATCCT Catgaccgag 1741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccAgggccgt acgcaccctc 1801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgaTccgga ccgccacatc 1861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 1921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 1981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 2041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 2101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 2161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 2221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 2281 Gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccATGCCC 2341 ACGCTACTGC GGGTTTATAT AGACGGTCCC CACGGGATGG GGAAAACCAC CACCACGCAA 2401 CTGCTGGTGG CCCTGGGTTC GCGCGACGAT ATCGTCTACG TACCCGAGCC GATGACTTAC 2461 TGGCGGGTGc TGGGGGCTTC CGAGACAATC GCGAACATCT ACACCACACA ACACCGCCTC 2521 GACCAGGGTG AGATATCGGC CGGGGACGCG GCGGTGGTAA TGACAAGCGC CCAGATAACA 2581 ATGGGCATGC CTTATGCCGT GACCGACGCC GTTCTGGCTC CTCATATCGG GGGGGAGGCT 2641 GGGAGCTCAC ATGCCCCGCC CCCGGCCCTC ACCCTCATCT TCGACCGCCA TCCCATCGCC 2701 GCCCTCCTGT GCTACCCGGC CGCGCGgTAC CTTATGGGCA GCATGACCCC CCAGGCCGTG 2761 CTGGCGTTCG TGGCCCTCAT CCCGCCGACC TTGCCCGGCA CcAACATCGT GcTTGGGGCC 2821 CTTCCGGAGG ACAGACACAT CGACCGCCTG GCCAAACGCC AGCGCCCCGG CGAGCGGCTg 2881 GACCTGGCTA TGCTGGCtGC GATTCGCCGC GTTTAcGGGC TaCTTGCCAA TACGGTGCGG 2941 TATCTGCAGt GCGGCGGGTC GTGGCGGGAG GAcTGGGGAC AGCTTTCGGG GaCGGCCGTG 3001 CCGCCCCAGG GTGCCGAGCC CCAGAGCAAC GCGGGCCCAC GACCCCATAT CGGGGACACG 3061 TTATTTACCC TGTTTCGGGC CCCCGAGTTG CTGGCCCCCA ACGGCGACCT GTATAACGTG 3121 TTTGCCTGGG CcTTGGACGT CTTGGCCAAA CGCCTCCGTT CCATGCACGT CTTTATCCTG 3181 GATTACGACC AATCGCCCGC CGGCTGCCGG GACGCCCTGC TGCAACTTAC CTCCGGGATG 3241 GTCCAGACCC ACGTCACCAC CCCCGGCTCC ATACCGACGA TATGCGACCT GGCGCGCACG 3301 TTTGCCCGGG AGATGGGGGA GGCTAACTGA gctctagctt atcgactcgc cgatagtgga 3361 aaccgacgcc ccagcactcg tccgagggca aaggaatagt cgagaaattg atgatctatt 3421 aaacaataaa gatgtccaca tggaagtttt tcctgtcata ctttgttaag aagggtgaga 3481 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga 3541 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca 3601 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca 3661 ctcatgatct atagatccTc tagaGAGCTC ACGGGGACAG CCCCCCCCCA AAGCCCCCAG 3721 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 3781 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 3841 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 3901 CACCTGGGGG ATACGGGGAA AAAGCTgtcg aCCTGCAGGC ATGCAAGCTT GGCGTAATCA 3961 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4021 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4081 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGTA ACTCGAGGGA 4141 TCCAAGCTgg CCgcACGTCT CCCTATCAGT GATAGAGAAG TCGACACGTC TCGAGCTCCC 4201 TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT CAGTGATAGA GAAGTCGACA 4261 CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA GAACGTCTCC CTATCAGTGA 4321 TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG AGAAGGTACG TCTAGAACGT 4381 CTCCCTATCA GTGATAGAGA AGTCGACACG TCTCGAGCTC CCTATCAGTG ATAGAGAAGG 4441 TACCCCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC 4501 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GTGGCGGCCA 4561 TCACAAGTTT GTACAAAAAA GCAGGCTctc gagccaccaa tggtggatat tatttttcat 4621 tatccttttc tgggtgctat gggtgatcat tccaagaaga agcccgggac ggccatgtgc 4681 gtgggctgcg ggagtcagat ccacgaccag tttatcctgc gggtgtcgcc cgacctcgag 4741 tggcacgcgg cctgcctcaa gtgtgccgag tgcagccagt acctggacga gacgtgcacg 4801 tgcttcgtga gagacgggaa gacctactgc aagcgggact acgtcaggct gttcggcatc 4861 aagtgcgcca agtgccaggt gggcttcagc agcagcgacc tggtgatgag ggcgcgggac 4921 agcgtgtacc acatcgagtg cttccgctgc tccgtgtgca gccgccagct gctgcctggg 4981 gacgagttct cgctgcggga gcacgagctg ctctgccgcg ccgaccacgg cctcctgctc 5041 gagcgcgccg cggccggcag cccgcgcagc cccggcccgc ttcccggcgc ccgcggcctg 5101 catctgcccg acgctgggtc gggccggcag cccgcgttgc gcccgcacgt gcacaagcag 5161 acggagaaga cgacccgcgt gcggactgtg ctgaacgaga agcagctgca cactctgcgg 5221 acctgctacg ccgccaaccc gcggcccgac gctctcatga aggagcagct ggtggagatg 5281 accggcctga gcccgcgggt catccgcgtc tggttccaga acaagcgctg caaggacaag 5341 aagaaatcca ttctcatgaa gcagctgcag cagcagcagc acagcgacaa gacgagcctt 5401 cagggactga ctgggacgcc cctggtggcg ggcagtccca tccgccatga gaacgccgtg 5461 cagggcagcg cagtggaggt gcagacgtac cagccgccgt ggaaggcgct cagcgagttt 5521 gccctccaga gcgacctgga ccaacccgcc ttccaacagc tggtctcctt ctccgagtcc 5581 ggctccctag gcaactcctc cggcagcgac gtgacctccc tgtcctcgca gctcccggac 5641 acccccaaca gtatggtgcc gagtcccgtg gagacgtgag cggccgcACC CAGCTTTCTT 5701 GTACAAAGTG GTGATggccG CTCTAGCTAG TCGATAAGCT AATTCCGCCC CCCCCCCCCC 5761 CCCCCCCTCA CCcTCCCCCC CCCctaacgt tactggccga agccgcttgg aataaggccg 5821 gtgagcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 5881 ccgaaacctg gccctgtctt cttgacgacg attcctaggg gtctttcccc tctcgccaaa 5941 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga 6001 caacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacatggata 6061 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc 6121 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt 6181 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttggttt 6241 gaaaaacacg atgataatat ggccacaacc atggaacagg gggatcagct taccatggcc 6301 aagatccctc ctaagaagaa gcgcaaagtc gaggatcccg tcgttttaca acgtcgtgac 6361 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 6421 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 6481 ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag 6541 tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac 6601 gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc gtttgttccc 6661 acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag 6721 gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac 6781 gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca 6841 tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag tgacggcagt 6901 tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg 6961 cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc 7021 agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg 7081 gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc 7141 ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg tctgaacgtc 7201 gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg 7261 cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg 7321 cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac 7381 cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat 7441 atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat 7501 ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt 7561 gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg 7621 atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc 7681 tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc 7741 aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc 7801 accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg 7861 ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt 7921 tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg 7981 tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg 8041 attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg 8101 ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca 8161 gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc 8221 atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg 8281 gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa 8341 ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg 8401 ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc 8461 gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac 8521 gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt 8581 tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa 8641 ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta 8701 agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat 8761 taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga tgcggtgctg 8821 attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc 8881 taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat 8941 acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta 9001 aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt 9061 gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac 9121 ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc 9181 cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg 9241 ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc 9301 gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg tcgctaccat 9361 taccagttgg tctggtgtca ggggatcccc cgggctgcag ccaatatggg atcggccatt 9421 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 9481 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 9541 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 9601 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 9661 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 9721 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 9781 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 9841 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 9901 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgat 9961 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 10021 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 10081 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 10141 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 10201 ttcttctgag gggatcaatt ctctagggct cgctgatcag cctcgactgt gccttctagt 10261 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 10321 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 10381 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 10441 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 10501 tcgatcctct agagtcgaGG GCTGCAGGAA TTCGATATCA AGCTCTAGCC AACAAGCTCG 10561 TCATCGCTTT GCAGAAGAGC AGAGAGGATA TGCTCATCGT CTAAAGAACT ACCCATTTTA 10621 TTATATATTA GTCACgatat ctataacaag aaaatatata tataataagt tatcacgtaa 10681 gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa 10741 gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac 10801 cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac 10861 agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaATGC ATgcgtcaat 10921 tttacgcaga ctatctttct agggttaaAt ttGGGCGGTC GAGGGACCTA ataacttcgt 10981 ataGCATACA ttatacgaac ggtaATTAAG GGTTCCGGAT CCCCATCAAG CTAATTgaag 11041 ttcctattcC gaagttccta ttcTCTAGAA Agtataggaa cttcCAGCTG GAAAtGATTT 11101 GCGCTTTACT CGACCTAAAC TTTAAACACG TCATAGAATC TTCGTTTGAC AAAAACCACA 11161 TTGTGGCCAA GCTGTGTGAC GCGACGCGCG CTAAAGAATG GCAAACCAAG TCGCGCGAGG 11221 TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTCCGAG CTTGGCGTAA TCATGGTCAT 11281 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 11341 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 11401 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 11461 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 11521 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 11581 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 11641 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 11701 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 11761 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 11821 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 11881 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 11941 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 12001 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 12061 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 12121 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 12181 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 12241 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 12301 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 12361 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 12421 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 12481 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 12541 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 12601 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 12661 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 12721 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 12781 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 12841 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 12901 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 12961 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 13021 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 13081 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 13141 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 13201 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 13261 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 13321 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 13381 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTG //