LOCUS 91276_PB_tet_PHS 13644 bp ds-DNA circular 25-JUL-2013 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from 90838 pORF-MKRN1(ORS09644) 652 to 2133 COMMENT Backbone from 90946 PB-tet-PH-lox66FRT2 6253 to 4570 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 6042..6052 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /clone_lib="HIP_Gateway221_closed" /focus /note="Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=#ffffff /ApEinfo_revcolor=#ffffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1205..1459 /label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 source 4581..4592 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /clone_lib="HIP_Gateway221_closed" /focus /note="Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE:(1) /ApEinfo_label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=#ffffff /ApEinfo_revcolor=#ffffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3331..3678 /label=polyA /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(463..531) /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6042..6042 /note="linker at the 3' end of the ORF" /label=linker at the 3' end of the ORF /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4581..4587 /note="attL1 site" /label=attL1 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1460..1711 /label=mouse PGK promoter(1) /ApEinfo_label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 707..941 /label=PB 3'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6043..6052 /note="attL2 site" /label=attL2 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 6776..6844 /label=LacZ alpha(1) /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3685..3926 /label=chicken 5'-HS4 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4588..4592 /note="linker at the 5' end of the ORF that includes ACC for minimal Kozak consensus" /label=linker at the 5' end of the ORF that includes ACC /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4446..4551 /label=mCMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4593..6041 /label=MKRN1 /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6099..6622 /label=IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4160..4437 /label=8xTetO2 element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(6053..6067) /label=AttR2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 602..619 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6624..10566 /label=b-geo /ApEinfo_fwdcolor=#800000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 618..645 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6687..9712 /label=LacZ /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1732..2328 /label=Pac /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10567..10873 /label=pA /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10991..11261 /label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11451..11470) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11488..11508) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(135..441) /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(11876..12558) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(3..458) /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding complement(11514..11536) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2335..3330 /label=DeltaTK /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(12656..13315) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11262..11303 /label=PB 5'LTR(1) /ApEinfo_label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4563..4570 /label=AttR1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG 661 TATTCACGAC AGCAGGCTGA ATAATAAAAA AATTAGAAAC TATTATttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaAA CATTCTCTCT 961 TTTACAAAAA TAAACTTATT TTGTACTTTA AAAACAGTCA TGTTGTATTA TAAAATAAGT 1021 AATTAGCTTA ACCTATACAT AATAGAAACA AATTATACTT ATTAGTCAGT CAGAAACAAC 1081 TTTGGCACAT ATCAATATTA TGCTCTCGTT ACTGGCGAAA GGGGGATGTG CTGCAAGGCG 1141 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA 1201 ATTCtaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 1261 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 1321 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 1381 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 1441 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 1501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 1561 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 1621 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 1681 ctcctcttcc tcatctccgg gcctttcgac cGATCATCAA GCTTGATCCT Catgaccgag 1741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccAgggccgt acgcaccctc 1801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgaTccgga ccgccacatc 1861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 1921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 1981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 2041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 2101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 2161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 2221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 2281 Gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccATGCCC 2341 ACGCTACTGC GGGTTTATAT AGACGGTCCC CACGGGATGG GGAAAACCAC CACCACGCAA 2401 CTGCTGGTGG CCCTGGGTTC GCGCGACGAT ATCGTCTACG TACCCGAGCC GATGACTTAC 2461 TGGCGGGTGc TGGGGGCTTC CGAGACAATC GCGAACATCT ACACCACACA ACACCGCCTC 2521 GACCAGGGTG AGATATCGGC CGGGGACGCG GCGGTGGTAA TGACAAGCGC CCAGATAACA 2581 ATGGGCATGC CTTATGCCGT GACCGACGCC GTTCTGGCTC CTCATATCGG GGGGGAGGCT 2641 GGGAGCTCAC ATGCCCCGCC CCCGGCCCTC ACCCTCATCT TCGACCGCCA TCCCATCGCC 2701 GCCCTCCTGT GCTACCCGGC CGCGCGgTAC CTTATGGGCA GCATGACCCC CCAGGCCGTG 2761 CTGGCGTTCG TGGCCCTCAT CCCGCCGACC TTGCCCGGCA CcAACATCGT GcTTGGGGCC 2821 CTTCCGGAGG ACAGACACAT CGACCGCCTG GCCAAACGCC AGCGCCCCGG CGAGCGGCTg 2881 GACCTGGCTA TGCTGGCtGC GATTCGCCGC GTTTAcGGGC TaCTTGCCAA TACGGTGCGG 2941 TATCTGCAGt GCGGCGGGTC GTGGCGGGAG GAcTGGGGAC AGCTTTCGGG GaCGGCCGTG 3001 CCGCCCCAGG GTGCCGAGCC CCAGAGCAAC GCGGGCCCAC GACCCCATAT CGGGGACACG 3061 TTATTTACCC TGTTTCGGGC CCCCGAGTTG CTGGCCCCCA ACGGCGACCT GTATAACGTG 3121 TTTGCCTGGG CcTTGGACGT CTTGGCCAAA CGCCTCCGTT CCATGCACGT CTTTATCCTG 3181 GATTACGACC AATCGCCCGC CGGCTGCCGG GACGCCCTGC TGCAACTTAC CTCCGGGATG 3241 GTCCAGACCC ACGTCACCAC CCCCGGCTCC ATACCGACGA TATGCGACCT GGCGCGCACG 3301 TTTGCCCGGG AGATGGGGGA GGCTAACTGA gctctagctt atcgactcgc cgatagtgga 3361 aaccgacgcc ccagcactcg tccgagggca aaggaatagt cgagaaattg atgatctatt 3421 aaacaataaa gatgtccaca tggaagtttt tcctgtcata ctttgttaag aagggtgaga 3481 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga 3541 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca 3601 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca 3661 ctcatgatct atagatccTc tagaGAGCTC ACGGGGACAG CCCCCCCCCA AAGCCCCCAG 3721 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 3781 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 3841 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 3901 CACCTGGGGG ATACGGGGAA AAAGCTgtcg aCCTGCAGGC ATGCAAGCTT GGCGTAATCA 3961 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4021 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4081 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGTA ACTCGAGGGA 4141 TCCAAGCTgg CCgcACGTCT CCCTATCAGT GATAGAGAAG TCGACACGTC TCGAGCTCCC 4201 TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT CAGTGATAGA GAAGTCGACA 4261 CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA GAACGTCTCC CTATCAGTGA 4321 TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG AGAAGGTACG TCTAGAACGT 4381 CTCCCTATCA GTGATAGAGA AGTCGACACG TCTCGAGCTC CCTATCAGTG ATAGAGAAGG 4441 TACCCCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC 4501 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GTGGCGGCCA 4561 TCACAAGTTT gtacaaaaaa gcaggctcca ccATGGCGGA GGCTGCAACT CCCGGAACAA 4621 CAGCCACAAC ATCAGGAGCA GGAGCGGCAG CGGCGACGGC GGCAGCAGCC TCCCCCACCC 4681 CGATCCCCAC AGTCACCGCC CCGTCCCTGG GGGCGGGCGG AGGGGGCGGC GGCAGCGACG 4741 GCAGCGGCGG CGGCTGGACT AAACAGGTCA CCTGCAGGTA TTTTATGCAT GGGGTTTGTA 4801 AGGAAGGAGA CAACTGTCGC TACTCGCATG ACCTCTCTGA CAGTCCGTAT AGTGTAGTGT 4861 GCAAGTATTT TCAGCGAGGG TACTGTATTT ATGGAGACCG CTGCAGATAT GAACATAGCA 4921 AACCATTGAA ACAGGAAGAA GCAACTGCTA CAGAGCTAAC TACAAAGTCA TCCCTTGCTG 4981 CTTCCTCAAG TCTCTCATCG ATAGTTGGAC CACTTGTTGA AATGAATACA GGCGAAGCTG 5041 AGTCAAGAAA TTCAAACTTT GCAACTGTAG GAGCAGGTTC AGAGGACTGG GTGAATGCTA 5101 TTGAGTTTGT TCCTGGGCAA CCCTACTGTG GCCGTACTGC GCCTTCCTGC ACTGAAGCAC 5161 CCCTGCAGGG CTCAGTGACC AAGGAAGAAT CAGAGAAAGA GCAAACCGCC GTGGAGACAA 5221 AGAAGCAGCT GTGCCCCTAT GCTGCAGTGG GAGAGTGCCG ATACGGGGAG AACTGTGTGT 5281 ATCTCCACGG AGATTCTTGT GACATGTGTG GGCTGCAGGT CCTGCATCCA ATGGATGCTG 5341 CCCAGAGATC GCAGCATATC AAATCGTGCA TTGAGGCCCA TGAGAAGGAC ATGGAGCTCT 5401 CATTTGCCGT GCAGCGCAGC AAGGACATGG TGTGTGGGAT CTGCATGGAG GTGGTCTATG 5461 AGAAAGCCAA CCCCAGTGAG CGCCGCTTCG GGATCCTCTC CAACTGCAAC CACACCTACT 5521 GTCTCAAGTG CATTCGCAAG TGGAGGAGTG CTAAGCAATT TGAGAGCAAG ATCATAAAGT 5581 CCTGCCCAGA ATGCCGGATC ACATCTAACT TTGTCATTCC AAGTGAGTAC TGGGTGGAGG 5641 AGAAAGAAGA GAAGCAGAAA CTCATTCTGA AATACAAGGA GGCAATGAGC AACAAGGCGT 5701 GCAGGTATTT TGATGAAGGA CGTGGGAGCT GCCCATTTGG AGGGAACTGT TTTTACAAGC 5761 ATGCGTACCC TGATGGCCGT AGAGAGGAGC CACAGAGACA GAAAGTGGGA ACATCAAGCA 5821 GATACCGGGC CCAACGAAGG AACCACTTCT GGGAACTCAT TGAGGAAAGA GAGAACAGCA 5881 ACCCCTTTGA CAACGATGAA GAAGAGGTTG TCACCTTTGA GCTGGGCGAG ATGTTGCTTA 5941 TGCTTTTGGC TGCAGGTGGG GACGACGAAC TAACAGACTC TGAAGATGAG TGGGACTTGT 6001 TTCATGATGA GCTGGAAGAT TTTTATGACT TGGATCTATA Ggacccagct ttCTTGTACA 6061 AAGTGGTGAT ggccGCTCTA GCTAGTCGAT AAGCTAATTC CGCCCCCCCC CCCCCCCCCC 6121 CCTCACCcTC CCCCCCCCct aacgttactg gccgaagccg cttggaataa ggccggtgag 6181 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgaa 6241 acctggccct gtcttcttga cgacgattcc taggggtctt tcccctctcg ccaaaggaat 6301 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaca 6361 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacat ggatagttgt 6421 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa 6481 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta 6541 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt ggtttgaaaa 6601 acacgatgat aatatggcca caaccatgga acagggggat cagcttacca tggccaagat 6661 ccctcctaag aagaagcgca aagtcgagga tcccgtcgtt ttacaacgtc gtgactggga 6721 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 6781 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 6841 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga 6901 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc 6961 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga 7021 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg 7081 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg 7141 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt 7201 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct 7261 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa 7321 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg 7381 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac 7441 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga 7501 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa 7561 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac 7621 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat 7681 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca 7741 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct 7801 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct 7861 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac 7921 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag 7981 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc 8041 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc 8101 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga 8161 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa 8221 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga 8281 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg 8341 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa 8401 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa 8461 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct 8521 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga 8581 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc 8641 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa 8701 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac 8761 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg 8821 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat 8881 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca 8941 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct 9001 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga 9061 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca 9121 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac 9181 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg 9241 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc 9301 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg 9361 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg 9421 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct 9481 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt 9541 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca 9601 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga 9661 ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca 9721 gttggtctgg tgtcagggga tcccccgggc tgcagccaat atgggatcgg ccattgaaca 9781 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 9841 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 9901 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 9961 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 10021 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 10081 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 10141 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 10201 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 10261 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct 10321 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 10381 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 10441 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 10501 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 10561 ctgaggggat caattctcta gggctcgctg atcagcctcg actgtgcctt ctagttgcca 10621 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 10681 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 10741 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 10801 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat 10861 cctctagagt cgaGGGCTGC AGGAATTCGA TATCAAGCTC TAGCCAACAA GCTCGTCATC 10921 GCTTTGCAGA AGAGCAGAGA GGATATGCTC ATCGTCTAAA GAACTACCCA TTTTATTATA 10981 TATTAGTCAC gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga 11041 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa 11101 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat 11161 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga 11221 cggattcgcg ctatttagaa agagagagca atatttcaag aATGCATgcg tcaattttac 11281 gcagactatc tttctagggt taaAtttAAA tGATTTGCGC TTTACTCGAC CTAAACTTTA 11341 AACACGTCAT AGAATCTTCG TTTGACAAAA ACCACATTGT GGCCAAGCTG TGTGACGCGA 11401 CGCGCGCTAA AGAATGGCAA ACCAAGTCGC GCGAGGTACC CAGCTTTTGT TCCCTTTAGT 11461 GAGGGTTAAT TCCGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 11521 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 11581 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 11641 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 11701 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 11761 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 11821 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 11881 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 11941 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 12001 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 12061 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 12121 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 12181 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 12241 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 12301 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 12361 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 12421 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 12481 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 12541 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 12601 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 12661 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 12721 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 12781 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 12841 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 12901 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 12961 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 13021 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 13081 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 13141 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 13201 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 13261 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 13321 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 13381 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 13441 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 13501 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 13561 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 13621 CATTTCCCCG AAAAGTGCCA CCTG //