LOCUS New_DNA 13519 bp ds-DNA circular 06-FEB-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from 91884 pORF-PA2G4_ORS02725 652 to 1869 COMMENT Backbone from 90946 PB-tet-PH-lox66FRT2 6253 to 4570 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 5778..5788 /mol_type="other DNA" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /db_xref="taxon:32630" /organism="synthetic construct" /clone_lib="HIP_Gateway221_closed /focus /note=Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1205..1459 /label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 source 4581..4592 /mol_type="other DNA" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /db_xref="taxon:32630" /organism="synthetic construct" /clone_lib="HIP_Gateway221_closed /focus /note=Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE:(1) /ApEinfo_label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3331..3678 /label=polyA /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(463..531) /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5778..5778 /note="linker at the 3' end of the ORF" /label=linker at the 3' end of the ORF /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1460..1711 /label=mouse PGK promoter(1) /ApEinfo_label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 707..941 /label=PB 3'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4593..5777 /label=PA2G4(BC001951) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 6512..6580 /label=LacZ alpha(1) /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4446..4551 /label=mCMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4581..4587 /note="attL1 site" /label=attL1 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5835..6358 /label=IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(11142..11175) /label=CoreFRT /ApEinfo_fwdcolor=#8080c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3685..3926 /label=chicken 5'-HS4 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5779..5788 /note="attL2 site" /label=attL2 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4588..4592 /note="linker at the 5' end of the ORF that includes ACC for minimal Kozak consensus" /label=linker at the 5' end of the ORF that includes ACC /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11062..11095 /label=lox66 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4160..4437 /label=8xTetO2 element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(5789..5803) /label=AttR2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 602..619 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11128..11175 /label=FRT /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 618..645 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6360..10302 /label=b-geo /ApEinfo_fwdcolor=#800000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1732..2328 /label=Pac /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6423..9448 /label=LacZ /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10303..10609 /label=pA /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11128..11140 /label=FRTunit /ApEinfo_fwdcolor=#80ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10727..10997 /label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11326..11345) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(135..441) /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11363..11383) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(3..458) /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(11751..12433) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2335..3330 /label=DeltaTK /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding complement(11389..11411) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(12531..13190) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4563..4570 /label=AttR1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10998..11039 /label=PB 5'LTR(1) /ApEinfo_label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG 661 TATTCACGAC AGCAGGCTGA ATAATAAAAA AATTAGAAAC TATTATttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaAA CATTCTCTCT 961 TTTACAAAAA TAAACTTATT TTGTACTTTA AAAACAGTCA TGTTGTATTA TAAAATAAGT 1021 AATTAGCTTA ACCTATACAT AATAGAAACA AATTATACTT ATTAGTCAGT CAGAAACAAC 1081 TTTGGCACAT ATCAATATTA TGCTCTCGTT ACTGGCGAAA GGGGGATGTG CTGCAAGGCG 1141 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA 1201 ATTCtaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 1261 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 1321 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 1381 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 1441 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 1501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 1561 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 1621 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 1681 ctcctcttcc tcatctccgg gcctttcgac cGATCATCAA GCTTGATCCT Catgaccgag 1741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccAgggccgt acgcaccctc 1801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgaTccgga ccgccacatc 1861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 1921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 1981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 2041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 2101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 2161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 2221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 2281 Gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccATGCCC 2341 ACGCTACTGC GGGTTTATAT AGACGGTCCC CACGGGATGG GGAAAACCAC CACCACGCAA 2401 CTGCTGGTGG CCCTGGGTTC GCGCGACGAT ATCGTCTACG TACCCGAGCC GATGACTTAC 2461 TGGCGGGTGc TGGGGGCTTC CGAGACAATC GCGAACATCT ACACCACACA ACACCGCCTC 2521 GACCAGGGTG AGATATCGGC CGGGGACGCG GCGGTGGTAA TGACAAGCGC CCAGATAACA 2581 ATGGGCATGC CTTATGCCGT GACCGACGCC GTTCTGGCTC CTCATATCGG GGGGGAGGCT 2641 GGGAGCTCAC ATGCCCCGCC CCCGGCCCTC ACCCTCATCT TCGACCGCCA TCCCATCGCC 2701 GCCCTCCTGT GCTACCCGGC CGCGCGgTAC CTTATGGGCA GCATGACCCC CCAGGCCGTG 2761 CTGGCGTTCG TGGCCCTCAT CCCGCCGACC TTGCCCGGCA CcAACATCGT GcTTGGGGCC 2821 CTTCCGGAGG ACAGACACAT CGACCGCCTG GCCAAACGCC AGCGCCCCGG CGAGCGGCTg 2881 GACCTGGCTA TGCTGGCtGC GATTCGCCGC GTTTAcGGGC TaCTTGCCAA TACGGTGCGG 2941 TATCTGCAGt GCGGCGGGTC GTGGCGGGAG GAcTGGGGAC AGCTTTCGGG GaCGGCCGTG 3001 CCGCCCCAGG GTGCCGAGCC CCAGAGCAAC GCGGGCCCAC GACCCCATAT CGGGGACACG 3061 TTATTTACCC TGTTTCGGGC CCCCGAGTTG CTGGCCCCCA ACGGCGACCT GTATAACGTG 3121 TTTGCCTGGG CcTTGGACGT CTTGGCCAAA CGCCTCCGTT CCATGCACGT CTTTATCCTG 3181 GATTACGACC AATCGCCCGC CGGCTGCCGG GACGCCCTGC TGCAACTTAC CTCCGGGATG 3241 GTCCAGACCC ACGTCACCAC CCCCGGCTCC ATACCGACGA TATGCGACCT GGCGCGCACG 3301 TTTGCCCGGG AGATGGGGGA GGCTAACTGA gctctagctt atcgactcgc cgatagtgga 3361 aaccgacgcc ccagcactcg tccgagggca aaggaatagt cgagaaattg atgatctatt 3421 aaacaataaa gatgtccaca tggaagtttt tcctgtcata ctttgttaag aagggtgaga 3481 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga 3541 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca 3601 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca 3661 ctcatgatct atagatccTc tagaGAGCTC ACGGGGACAG CCCCCCCCCA AAGCCCCCAG 3721 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 3781 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 3841 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 3901 CACCTGGGGG ATACGGGGAA AAAGCTgtcg aCCTGCAGGC ATGCAAGCTT GGCGTAATCA 3961 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4021 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4081 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGTA ACTCGAGGGA 4141 TCCAAGCTgg CCgcACGTCT CCCTATCAGT GATAGAGAAG TCGACACGTC TCGAGCTCCC 4201 TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT CAGTGATAGA GAAGTCGACA 4261 CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA GAACGTCTCC CTATCAGTGA 4321 TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG AGAAGGTACG TCTAGAACGT 4381 CTCCCTATCA GTGATAGAGA AGTCGACACG TCTCGAGCTC CCTATCAGTG ATAGAGAAGG 4441 TACCCCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC 4501 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GTGGCGGCCA 4561 TCACAAGTTT gtacaaaaaa gcaggctcca ccatgtcggg cgaggacgag caacaggagc 4621 aaactatcgc tgaggacctg gtcgtgacca agtataagat ggggggcgac atcgccaaca 4681 gggtacttcg gtccttggtg gaagcatcta gctcaggtgt gtcggtactg agcctgtgtg 4741 agaaaggtga tgccatgatt atggaagaaa cagggaaaat cttcaagaaa gaaaaggaaa 4801 tgaagaaagg tattgctttt cccaccagca tttcggtaaa taactgtgta tgtcacttct 4861 cccctttgaa gagcgaccag gattatattc tcaaggaagg tgacttggta aaaattgacc 4921 ttggggtcca tgtggatggc ttcatcgcta atgtagctca cacttttgtg gttgatgtag 4981 ctcaggggac ccaagtaaca gggaggaaag cagatgttat taaggcagct cacctttgtg 5041 ctgaagctgc cctacgcctg gtcaaacctg gaaatcagaa cacacaagtg acagaagcct 5101 ggaacaaagt tgcccactca tttaactgca cgccaataga aggtatgctg tcacaccagt 5161 tgaagcagca tgtcatcgat ggagaaaaaa ccattatcca gaatcccaca gaccagcaga 5221 agaaggacca tgaaaaagct gaatttgagg tacatgaagt atatgctgtg gatgttctcg 5281 tcagctcagg agagggcaag gccaaggatg caggacagag aaccactatt tacaaacgag 5341 acccctctaa acagtatgga ctgaaaatga aaacttcacg tgccttcttc agtgaggtgg 5401 aaaggcgttt tgatgccatg ccgtttactt taagagcatt tgaagatgag aagaaggctc 5461 ggatgggtgt ggtggagtgc gccaaacatg aactgctgca accatttaat gttctctatg 5521 agaaggaggg tgaatttgtt gcccagttta aatttacagt tctgctcatg cccaatggcc 5581 ccatgcggat aaccagtggt cccttcgagc ctgacctcta caagtctgag atggaggtcc 5641 aggatgcaga gctaaaggcc ctcctccaga gttctgcaag tcgaaaaacc cagaaaaaga 5701 aaaaaaagaa ggcctccaag actgcagaga atgccaccag tggggaaaca ttagaagaaa 5761 atgaagctgg ggactaggac ccagctttCT TGTACAAAGT GGTGATggcc GCTCTAGCTA 5821 GTCGATAAGC TAATTCCGCC CCCCCCCCCC CCCCCCCCTC ACCcTCCCCC CCCCctaacg 5881 ttactggccg aagccgcttg gaataaggcc ggtgagcgtt tgtctatatg ttattttcca 5941 ccatattgcc gtcttttggc aatgtgaggg cccgaaacct ggccctgtct tcttgacgac 6001 gattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa 6061 ggaagcagtt cctctggaag cttcttgaag acaacaacgt ctgtagcgac cctttgcagg 6121 cagcggaacc ccccacctgg cgacatggat agttgtggaa agagtcaaat ggctctcctc 6181 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga 6241 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc 6301 cccccgaacc acggggacgt ggttttggtt tgaaaaacac gatgataata tggccacaac 6361 catggaacag ggggatcagc ttaccatggc caagatccct cctaagaaga agcgcaaagt 6421 cgaggatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 6481 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 6541 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc 6601 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt 6661 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta 6721 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct 6781 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg 6841 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag 6901 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc 6961 ggtgatggtg ctgcgttgga gtgacggcag ttatctggaa gatcaggata tgtggcggat 7021 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt 7081 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca 7141 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac 7201 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta 7261 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat 7321 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc 7381 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa 7441 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca 7501 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa 7561 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta 7621 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg 7681 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca 7741 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg 7801 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca 7861 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg 7921 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc 7981 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag 8041 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg 8101 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg 8161 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg 8221 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca 8281 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg 8341 tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag 8401 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact 8461 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc 8521 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa 8581 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat 8641 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct 8701 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac 8761 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc 8821 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg 8881 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca 8941 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc 9001 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa 9061 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa 9121 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat 9181 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa 9241 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca 9301 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa 9361 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc 9421 ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aggggatccc 9481 ccgggctgca gccaatatgg gatcggccat tgaacaagat ggattgcacg caggttctcc 9541 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 9601 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 9661 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac 9721 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 9781 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 9841 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 9901 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 9961 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 10021 caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg 10081 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 10141 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 10201 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 10261 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatcaat tctctagggc 10321 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 10381 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 10441 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 10501 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 10561 ggcttctgag gcggaaagaa ccagctgggg ctcgatcctc tagagtcgaG GGCTGCAGGA 10621 ATTCGATATC AAGCTCTAGC CAACAAGCTC GTCATCGCTT TGCAGAAGAG CAGAGAGGAT 10681 ATGCTCATCG TCTAAAGAAC TACCCATTTT ATTATATATT AGTCACgata tctataacaa 10741 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc 10801 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg 10861 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct 10921 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag 10981 agagcaatat ttcaagaATG CATgcgtcaa ttttacgcag actatctttc tagggttaaA 11041 tttGGGCGGT CGAGGGACCT Aataacttcg tataGCATAC Attatacgaa cggtaATTAA 11101 GGGTTCCGGA TCCCCATCAA GCTAATTgaa gttcctattc Cgaagttcct attcTCTAGA 11161 AAgtatagga acttcCAGCT GGAAAtGATT TGCGCTTTAC TCGACCTAAA CTTTAAACAC 11221 GTCATAGAAT CTTCGTTTGA CAAAAACCAC ATTGTGGCCA AGCTGTGTGA CGCGACGCGC 11281 GCTAAAGAAT GGCAAACCAA GTCGCGCGAG GTACCCAGCT TTTGTTCCCT TTAGTGAGGG 11341 TTAATTCCGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 11401 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 11461 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 11521 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 11581 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 11641 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 11701 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 11761 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 11821 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 11881 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 11941 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 12001 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 12061 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 12121 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 12181 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 12241 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 12301 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 12361 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 12421 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 12481 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 12541 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 12601 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 12661 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 12721 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 12781 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 12841 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 12901 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 12961 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 13021 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 13081 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 13141 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 13201 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 13261 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 13321 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 13381 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 13441 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 13501 CCCCGAAAAG TGCCACCTG //