LOCUS New_DNA 13854 bp ds-DNA circular 01-NOV-2013 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from 90791 pORF-SMAD4 652 to 2343 COMMENT Backbone from 90898 PB-tet-PH+SwaI 6253 to 4570 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 6252..6262 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /clone_lib="HIP_Gateway221_closed" /focus /note="Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=#ffffff /ApEinfo_revcolor=#ffffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1205..1459 /label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 source 4581..4592 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /clone_lib="HIP_Gateway221_closed" /focus /note="Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE:(1) /ApEinfo_label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=#ffffff /ApEinfo_revcolor=#ffffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3331..3678 /label=polyA /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(463..531) /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6252..6252 /note="linker at the 3' end of the ORF" /label=linker at the 3' end of the ORF /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1460..1711 /label=mouse PGK promoter(1) /ApEinfo_label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 707..941 /label=PB 3'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4581..4587 /note="attL1 site" /label=attL1 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 6986..7054 /label=LacZ alpha(1) /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4160..4437 /label=8xTetO2 element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6253..6262 /note="attL2 site" /label=attL2 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6309..6832 /label=IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3685..3926 /label=chicken 5'-HS4 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4588..4592 /note="linker at the 5' end of the ORF that includes ACC for minimal Kozak consensus" /label=linker at the 5' end of the ORF that includes ACC /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4593..6251 /label=SMAD4 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4446..4551 /label=mCMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(6263..6277) /label=AttR2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 602..619 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6834..10776 /label=b-geo /ApEinfo_fwdcolor=#800000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 618..645 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6897..9922 /label=LacZ /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1732..2328 /label=Pac /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10777..11083 /label=pA /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11201..11471 /label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11661..11680) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11698..11718) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(135..441) /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(12086..12768) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(3..458) /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding complement(11724..11746) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2335..3330 /label=DeltaTK /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(12866..13525) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11472..11513 /label=PB 5'LTR(1) /ApEinfo_label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4563..4570 /label=AttR1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG 661 TATTCACGAC AGCAGGCTGA ATAATAAAAA AATTAGAAAC TATTATttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaAA CATTCTCTCT 961 TTTACAAAAA TAAACTTATT TTGTACTTTA AAAACAGTCA TGTTGTATTA TAAAATAAGT 1021 AATTAGCTTA ACCTATACAT AATAGAAACA AATTATACTT ATTAGTCAGT CAGAAACAAC 1081 TTTGGCACAT ATCAATATTA TGCTCTCGTT ACTGGCGAAA GGGGGATGTG CTGCAAGGCG 1141 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA 1201 ATTCtaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 1261 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 1321 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 1381 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 1441 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 1501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 1561 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 1621 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 1681 ctcctcttcc tcatctccgg gcctttcgac cGATCATCAA GCTTGATCCT Catgaccgag 1741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccAgggccgt acgcaccctc 1801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgaTccgga ccgccacatc 1861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 1921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 1981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 2041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 2101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 2161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 2221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 2281 Gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccATGCCC 2341 ACGCTACTGC GGGTTTATAT AGACGGTCCC CACGGGATGG GGAAAACCAC CACCACGCAA 2401 CTGCTGGTGG CCCTGGGTTC GCGCGACGAT ATCGTCTACG TACCCGAGCC GATGACTTAC 2461 TGGCGGGTGc TGGGGGCTTC CGAGACAATC GCGAACATCT ACACCACACA ACACCGCCTC 2521 GACCAGGGTG AGATATCGGC CGGGGACGCG GCGGTGGTAA TGACAAGCGC CCAGATAACA 2581 ATGGGCATGC CTTATGCCGT GACCGACGCC GTTCTGGCTC CTCATATCGG GGGGGAGGCT 2641 GGGAGCTCAC ATGCCCCGCC CCCGGCCCTC ACCCTCATCT TCGACCGCCA TCCCATCGCC 2701 GCCCTCCTGT GCTACCCGGC CGCGCGgTAC CTTATGGGCA GCATGACCCC CCAGGCCGTG 2761 CTGGCGTTCG TGGCCCTCAT CCCGCCGACC TTGCCCGGCA CcAACATCGT GcTTGGGGCC 2821 CTTCCGGAGG ACAGACACAT CGACCGCCTG GCCAAACGCC AGCGCCCCGG CGAGCGGCTg 2881 GACCTGGCTA TGCTGGCtGC GATTCGCCGC GTTTAcGGGC TaCTTGCCAA TACGGTGCGG 2941 TATCTGCAGt GCGGCGGGTC GTGGCGGGAG GAcTGGGGAC AGCTTTCGGG GaCGGCCGTG 3001 CCGCCCCAGG GTGCCGAGCC CCAGAGCAAC GCGGGCCCAC GACCCCATAT CGGGGACACG 3061 TTATTTACCC TGTTTCGGGC CCCCGAGTTG CTGGCCCCCA ACGGCGACCT GTATAACGTG 3121 TTTGCCTGGG CcTTGGACGT CTTGGCCAAA CGCCTCCGTT CCATGCACGT CTTTATCCTG 3181 GATTACGACC AATCGCCCGC CGGCTGCCGG GACGCCCTGC TGCAACTTAC CTCCGGGATG 3241 GTCCAGACCC ACGTCACCAC CCCCGGCTCC ATACCGACGA TATGCGACCT GGCGCGCACG 3301 TTTGCCCGGG AGATGGGGGA GGCTAACTGA gctctagctt atcgactcgc cgatagtgga 3361 aaccgacgcc ccagcactcg tccgagggca aaggaatagt cgagaaattg atgatctatt 3421 aaacaataaa gatgtccaca tggaagtttt tcctgtcata ctttgttaag aagggtgaga 3481 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga 3541 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca 3601 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca 3661 ctcatgatct atagatccTc tagaGAGCTC ACGGGGACAG CCCCCCCCCA AAGCCCCCAG 3721 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 3781 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 3841 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 3901 CACCTGGGGG ATACGGGGAA AAAGCTgtcg aCCTGCAGGC ATGCAAGCTT GGCGTAATCA 3961 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4021 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4081 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGTA ACTCGAGGGA 4141 TCCAAGCTgg CCgcACGTCT CCCTATCAGT GATAGAGAAG TCGACACGTC TCGAGCTCCC 4201 TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT CAGTGATAGA GAAGTCGACA 4261 CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA GAACGTCTCC CTATCAGTGA 4321 TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG AGAAGGTACG TCTAGAACGT 4381 CTCCCTATCA GTGATAGAGA AGTCGACACG TCTCGAGCTC CCTATCAGTG ATAGAGAAGG 4441 TACCCCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC 4501 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GTGGCGGCCA 4561 TCACAAGTTT gtacaaaaaa gcaggctcca ccATGGACAA TATGTCTATT ACGAATACAC 4621 CAACAAGTAA TGATGCCTGT CTGAGCATTG TGCATAGTTT GATGTGCCAT AGACAAGGTG 4681 GAGAGAGTGA AACATTTGCA AAAAGAGCAA TTGAAAGTTT GGTAAAGAAG CTGAAGGAGA 4741 AAAAAGATGA ATTGGATTCT TTAATAACAG CTATAACTAC AAATGGAGCT CATCCTAGTA 4801 AATGTGTTAC CATACAGAGA ACATTGGATG GGAGGCTTCA GGTGGCTGGT CGGAAAGGAT 4861 TTCCTCATGT GATCTATGCC CGTCTCTGGA GGTGGCCTGA TCTTCACAAA AATGAACTAA 4921 AACATGTTAA ATATTGTCAG TATGCGTTTG ACTTAAAATG TGATAGTGTC TGTGTGAATC 4981 CATATCACTA CGAACGAGTT GTATCACCTG GAATTGATCT CTCAGGATTA ACACTGCAGA 5041 GTAATGCTCC ATCAAGTATG ATGGTGAAGG ATGAATATGT GCATGACTTT GAGGGACAGC 5101 CATCGTTGTC CACTGAAGGA CATTCAATTC AAACCATCCA GCATCCACCA AGTAATCGTG 5161 CATCGACAGA GACATACAGC ACCCCAGCTC TGTTAGCCCC ATCTGAGTCT AATGCTACCA 5221 GCACTGCCAA CTTTCCCAAC ATTCCTGTGG CTTCCACAAG TCAGCCTGCC AGTATACTGG 5281 GGGGCAGCCA TAGTGAAGGA CTGTTGCAGA TAGCATCAGG GCCTCAGCCA GGACAGCAGC 5341 AGAATGGATT TACTGGTCAG CCAGCTACTT ACCATCATAA CAGCACTACC ACCTGGACTG 5401 GAAGTAGGAC TGCACCATAC ACACCTAATT TGCCTCACCA CCAAAACGGC CATCTTCAGC 5461 ACCACCCGCC TATGCCGCCC CATCCCGGAC ATTACTGGCC TGTTCACAAT GAGCTTGCAT 5521 TCCAGCCTCC CATTTCCAAT CATCCTGCTC CTGAGTATTG GTGTTCCATT GCTTACTTTG 5581 AAATGGATGT TCAGGTAGGA GAGACATTTA AGGTTCCTTC AAGCTGCCCT ATTGTTACTG 5641 TTGATGGATA CGTGGACCCT TCTGGAGGAG ATCGCTTTTG TTTGGGTCAA CTCTCCAATG 5701 TCCACAGGAC AGAAGCCATT GAGAGAGCAA GGTTGCACAT AGGCAAAGGT GTGCAGTTGG 5761 AATGTAAAGG TGAAGGTGAT GTTTGGGTCA GGTGCCTTAG TGACCACGCG GTCTTTGTAC 5821 AGAGTTACTA CTTAGACAGA GAAGCTGGGC GTGCACCTGG AGATGCTGTT CATAAGATCT 5881 ACCCAAGTGC ATATATAAAG GTCTTTGATT TGCGTCAGTG TCATCGACAG ATGCAGCAGC 5941 AGGCGGCTAC TGCACAAGCT GCAGCAGCTG CCCAGGCAGC AGCCGTGGCA GGAAACATCC 6001 CTGGCCCAGG ATCAGTAGGT GGAATAGCTC CAGCTATCAG TCTGTCAGCT GCTGCTGGAA 6061 TTGGTGTTGA TGACCTTCGT CGCTTATGCA TACTCAGGAT GAGTTTTGTG AAAGGCTGGG 6121 GACCGGATTA CCCAAGACAG AGCATCAAAG AAACACCTTG CTGGATTGAA ATTCACTTAC 6181 ACCGGGCCCT CCAGCTCCTA GACGAAGTAC TTCATACCAT GCCGATTGCA GACCCACAAC 6241 CTTTAGACTA Ggacccagct ttCTTGTACA AAGTGGTGAT ggccGCTCTA GCTAGTCGAT 6301 AAGCTAATTC CGCCCCCCCC CCCCCCCCCC CCTCACCcTC CCCCCCCCct aacgttactg 6361 gccgaagccg cttggaataa ggccggtgag cgtttgtcta tatgttattt tccaccatat 6421 tgccgtcttt tggcaatgtg agggcccgaa acctggccct gtcttcttga cgacgattcc 6481 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 6541 agttcctctg gaagcttctt gaagacaaca acgtctgtag cgaccctttg caggcagcgg 6601 aaccccccac ctggcgacat ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 6661 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 6721 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg 6781 aaccacgggg acgtggtttt ggtttgaaaa acacgatgat aatatggcca caaccatgga 6841 acagggggat cagcttacca tggccaagat ccctcctaag aagaagcgca aagtcgagga 6901 tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 6961 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 7021 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga 7081 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc 7141 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat 7201 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt 7261 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa 7321 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt 7381 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat 7441 ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg 7501 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt 7561 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg 7621 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt 7681 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga 7741 tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa 7801 tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc 7861 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa 7921 gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat 7981 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt 8041 gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct 8101 gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac 8161 cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga 8221 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa 8281 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga 8341 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga 8401 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc 8461 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg 8521 cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt 8581 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc 8641 ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt 8701 ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt 8761 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag 8821 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga 8881 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca 8941 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc 9001 atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag 9061 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt 9121 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca 9181 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc 9241 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt 9301 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc 9361 agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa 9421 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac 9481 cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca 9541 gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc 9601 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac 9661 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg 9721 cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact 9781 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga 9841 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt 9901 ccagctgagc gccggtcgct accattacca gttggtctgg tgtcagggga tcccccgggc 9961 tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc 10021 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 10081 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 10141 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 10201 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 10261 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 10321 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 10381 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 10441 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 10501 caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc 10561 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 10621 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 10681 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 10741 cgccttctat cgccttcttg acgagttctt ctgaggggat caattctcta gggctcgctg 10801 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 10861 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 10921 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 10981 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 11041 tgaggcggaa agaaccagct ggggctcgat cctctagagt cgaGGGCTGC AGGAATTCGA 11101 TATCAAGCTC TAGCCAACAA GCTCGTCATC GCTTTGCAGA AGAGCAGAGA GGATATGCTC 11161 ATCGTCTAAA GAACTACCCA TTTTATTATA TATTAGTCAC gatatctata acaagaaaat 11221 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga 11281 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt 11341 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc 11401 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca 11461 atatttcaag aATGCATgcg tcaattttac gcagactatc tttctagggt taaAtttAAA 11521 tGATTTGCGC TTTACTCGAC CTAAACTTTA AACACGTCAT AGAATCTTCG TTTGACAAAA 11581 ACCACATTGT GGCCAAGCTG TGTGACGCGA CGCGCGCTAA AGAATGGCAA ACCAAGTCGC 11641 GCGAGGTACC CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT TCCGAGCTTG GCGTAATCAT 11701 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 11761 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 11821 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 11881 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 11941 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 12001 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 12061 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 12121 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 12181 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 12241 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 12301 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 12361 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 12421 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 12481 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 12541 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 12601 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 12661 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 12721 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 12781 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 12841 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 12901 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 12961 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 13021 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 13081 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 13141 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 13201 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 13261 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 13321 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 13381 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 13441 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 13501 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 13561 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 13621 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 13681 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 13741 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 13801 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTG //