LOCUS New_DNA 12817 bp ds-DNA circular 06-FEB-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from 91975 pORF-TWIST2_ORS09473 652 to 1167 COMMENT Backbone from 90946 PB-tet-PH-lox66FRT2 6253 to 4570 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 5076..5086 /mol_type="other DNA" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /db_xref="taxon:32630" /organism="synthetic construct" /clone_lib="HIP_Gateway221_closed /focus /note=Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=#ffffff /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1205..1459 /label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 source 4581..4592 /mol_type="other DNA" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /db_xref="taxon:32630" /organism="synthetic construct" /clone_lib="HIP_Gateway221_closed /focus /note=Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE:(1) /ApEinfo_label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=#ffffff /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3331..3678 /label=polyA /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(463..531) /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5076..5076 /note="linker at the 3' end of the ORF" /label=linker at the 3' end of the ORF /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1460..1711 /label=mouse PGK promoter(1) /ApEinfo_label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 707..941 /label=PB 3'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4593..5075 /label=TWIST2(BC033168) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 5810..5878 /label=LacZ alpha(1) /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4446..4551 /label=mCMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4581..4587 /note="attL1 site" /label=attL1 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5133..5656 /label=IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(10440..10473) /label=CoreFRT /ApEinfo_fwdcolor=#8080c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3685..3926 /label=chicken 5'-HS4 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5077..5086 /note="attL2 site" /label=attL2 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4588..4592 /note="linker at the 5' end of the ORF that includes ACC for minimal Kozak consensus" /label=linker at the 5' end of the ORF that includes ACC /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10360..10393 /label=lox66 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4160..4437 /label=8xTetO2 element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(5087..5101) /label=AttR2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 602..619 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10426..10473 /label=FRT /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 618..645 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5658..9600 /label=b-geo /ApEinfo_fwdcolor=#800000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1732..2328 /label=Pac /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5721..8746 /label=LacZ /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 9601..9907 /label=pA /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10426..10438 /label=FRTunit /ApEinfo_fwdcolor=#80ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10025..10295 /label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(10624..10643) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(135..441) /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(10661..10681) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(3..458) /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(11049..11731) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2335..3330 /label=DeltaTK /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding complement(10687..10709) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(11829..12488) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4563..4570 /label=AttR1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10296..10337 /label=PB 5'LTR(1) /ApEinfo_label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG 661 TATTCACGAC AGCAGGCTGA ATAATAAAAA AATTAGAAAC TATTATttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaAA CATTCTCTCT 961 TTTACAAAAA TAAACTTATT TTGTACTTTA AAAACAGTCA TGTTGTATTA TAAAATAAGT 1021 AATTAGCTTA ACCTATACAT AATAGAAACA AATTATACTT ATTAGTCAGT CAGAAACAAC 1081 TTTGGCACAT ATCAATATTA TGCTCTCGTT ACTGGCGAAA GGGGGATGTG CTGCAAGGCG 1141 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA 1201 ATTCtaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 1261 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 1321 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 1381 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 1441 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 1501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 1561 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 1621 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 1681 ctcctcttcc tcatctccgg gcctttcgac cGATCATCAA GCTTGATCCT Catgaccgag 1741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccAgggccgt acgcaccctc 1801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgaTccgga ccgccacatc 1861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 1921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 1981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 2041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 2101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 2161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 2221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 2281 Gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccATGCCC 2341 ACGCTACTGC GGGTTTATAT AGACGGTCCC CACGGGATGG GGAAAACCAC CACCACGCAA 2401 CTGCTGGTGG CCCTGGGTTC GCGCGACGAT ATCGTCTACG TACCCGAGCC GATGACTTAC 2461 TGGCGGGTGc TGGGGGCTTC CGAGACAATC GCGAACATCT ACACCACACA ACACCGCCTC 2521 GACCAGGGTG AGATATCGGC CGGGGACGCG GCGGTGGTAA TGACAAGCGC CCAGATAACA 2581 ATGGGCATGC CTTATGCCGT GACCGACGCC GTTCTGGCTC CTCATATCGG GGGGGAGGCT 2641 GGGAGCTCAC ATGCCCCGCC CCCGGCCCTC ACCCTCATCT TCGACCGCCA TCCCATCGCC 2701 GCCCTCCTGT GCTACCCGGC CGCGCGgTAC CTTATGGGCA GCATGACCCC CCAGGCCGTG 2761 CTGGCGTTCG TGGCCCTCAT CCCGCCGACC TTGCCCGGCA CcAACATCGT GcTTGGGGCC 2821 CTTCCGGAGG ACAGACACAT CGACCGCCTG GCCAAACGCC AGCGCCCCGG CGAGCGGCTg 2881 GACCTGGCTA TGCTGGCtGC GATTCGCCGC GTTTAcGGGC TaCTTGCCAA TACGGTGCGG 2941 TATCTGCAGt GCGGCGGGTC GTGGCGGGAG GAcTGGGGAC AGCTTTCGGG GaCGGCCGTG 3001 CCGCCCCAGG GTGCCGAGCC CCAGAGCAAC GCGGGCCCAC GACCCCATAT CGGGGACACG 3061 TTATTTACCC TGTTTCGGGC CCCCGAGTTG CTGGCCCCCA ACGGCGACCT GTATAACGTG 3121 TTTGCCTGGG CcTTGGACGT CTTGGCCAAA CGCCTCCGTT CCATGCACGT CTTTATCCTG 3181 GATTACGACC AATCGCCCGC CGGCTGCCGG GACGCCCTGC TGCAACTTAC CTCCGGGATG 3241 GTCCAGACCC ACGTCACCAC CCCCGGCTCC ATACCGACGA TATGCGACCT GGCGCGCACG 3301 TTTGCCCGGG AGATGGGGGA GGCTAACTGA gctctagctt atcgactcgc cgatagtgga 3361 aaccgacgcc ccagcactcg tccgagggca aaggaatagt cgagaaattg atgatctatt 3421 aaacaataaa gatgtccaca tggaagtttt tcctgtcata ctttgttaag aagggtgaga 3481 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga 3541 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca 3601 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca 3661 ctcatgatct atagatccTc tagaGAGCTC ACGGGGACAG CCCCCCCCCA AAGCCCCCAG 3721 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 3781 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 3841 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 3901 CACCTGGGGG ATACGGGGAA AAAGCTgtcg aCCTGCAGGC ATGCAAGCTT GGCGTAATCA 3961 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4021 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4081 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGTA ACTCGAGGGA 4141 TCCAAGCTgg CCgcACGTCT CCCTATCAGT GATAGAGAAG TCGACACGTC TCGAGCTCCC 4201 TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT CAGTGATAGA GAAGTCGACA 4261 CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA GAACGTCTCC CTATCAGTGA 4321 TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG AGAAGGTACG TCTAGAACGT 4381 CTCCCTATCA GTGATAGAGA AGTCGACACG TCTCGAGCTC CCTATCAGTG ATAGAGAAGG 4441 TACCCCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC 4501 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GTGGCGGCCA 4561 TCACAAGTTT gtacaaaaaa gcaggctcca ccatggagga gggctccagc tcgcccgtgt 4621 cccccgtgga cagcctgggc accagcgagg aggagctcga gaggcagccc aagcgcttcg 4681 gccggaagcg gcgctacagc aagaagtcga gcgaagatgg cagcccgacc ccgggcaagc 4741 gcggcaagaa gggcagcccc agcgcgcagt ccttcgagga gctgcagagc cagcgcatcc 4801 tggccaacgt gcgcgagcgc cagcgcaccc agtcgctcaa cgaggccttc gcggcgctgc 4861 gcaagatcat ccccacgctg ccctctgaca agctgagcaa gatccagacg ctcaagctgg 4921 ccgccaggta catagacttc ctctaccagg tcctgcagag cgacgagatg gacaataaga 4981 tgaccagctg cagctacgtg gcccacgagc gcctcagcta cgccttctcc gtgtggcgca 5041 tggagggcgc gtggtccatg tccgcctccc actaggaccc agctttCTTG TACAAAGTGG 5101 TGATggccGC TCTAGCTAGT CGATAAGCTA ATTCCGCCCC CCCCCCCCCC CCCCCCTCAC 5161 CcTCCCCCCC CCctaacgtt actggccgaa gccgcttgga ataaggccgg tgagcgtttg 5221 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cgaaacctgg 5281 ccctgtcttc ttgacgacga ttcctagggg tctttcccct ctcgccaaag gaatgcaagg 5341 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aacaacgtct 5401 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acatggatag ttgtggaaag 5461 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc 5521 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag 5581 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttggtttg aaaaacacga 5641 tgataatatg gccacaacca tggaacaggg ggatcagctt accatggcca agatccctcc 5701 taagaagaag cgcaaagtcg aggatcccgt cgttttacaa cgtcgtgact gggaaaaccc 5761 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 5821 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg 5881 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc 5941 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat 6001 ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc 6061 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac 6121 gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt 6181 cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc 6241 cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt atctggaaga 6301 tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac 6361 tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt 6421 actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc 6481 tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat 6541 cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa 6601 actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga 6661 cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa 6721 tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca 6781 tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat 6841 gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta 6901 cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg 6961 catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg 7021 cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg 7081 gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga 7141 tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat 7201 ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc 7261 catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc 7321 ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta 7381 tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga 7441 tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg 7501 ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga 7561 agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac 7621 cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga 7681 tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt 7741 gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg 7801 cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca 7861 gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca 7921 tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa 7981 ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac tgctgacgcc 8041 gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac 8101 ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga 8161 agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc 8221 tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga 8281 tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc 8341 ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg 8401 attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga 8461 tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg 8521 cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaacat 8581 cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc tgcacgcgga 8641 agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg 8701 gagcccgtca gtatcggcgg aattccagct gagcgccggt cgctaccatt accagttggt 8761 ctggtgtcag gggatccccc gggctgcagc caatatggga tcggccattg aacaagatgg 8821 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 8881 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 8941 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 9001 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 9061 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 9121 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 9181 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 9241 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 9301 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt 9361 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 9421 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 9481 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 9541 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg 9601 ggatcaattc tctagggctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 9661 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 9721 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 9781 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 9841 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgatcctcta 9901 gagtcgaGGG CTGCAGGAAT TCGATATCAA GCTCTAGCCA ACAAGCTCGT CATCGCTTTG 9961 CAGAAGAGCA GAGAGGATAT GCTCATCGTC TAAAGAACTA CCCATTTTAT TATATATTAG 10021 TCACgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga 10081 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc 10141 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa 10201 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt 10261 cgcgctattt agaaagagag agcaatattt caagaATGCA Tgcgtcaatt ttacgcagac 10321 tatctttcta gggttaaAtt tGGGCGGTCG AGGGACCTAa taacttcgta taGCATACAt 10381 tatacgaacg gtaATTAAGG GTTCCGGATC CCCATCAAGC TAATTgaagt tcctattcCg 10441 aagttcctat tcTCTAGAAA gtataggaac ttcCAGCTGG AAAtGATTTG CGCTTTACTC 10501 GACCTAAACT TTAAACACGT CATAGAATCT TCGTTTGACA AAAACCACAT TGTGGCCAAG 10561 CTGTGTGACG CGACGCGCGC TAAAGAATGG CAAACCAAGT CGCGCGAGGT ACCCAGCTTT 10621 TGTTCCCTTT AGTGAGGGTT AATTCCGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 10681 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 10741 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 10801 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 10861 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 10921 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 10981 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 11041 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 11101 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 11161 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 11221 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 11281 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 11341 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 11401 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 11461 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT 11521 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 11581 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 11641 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 11701 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 11761 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 11821 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 11881 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 11941 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 12001 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 12061 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 12121 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 12181 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 12241 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 12301 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 12361 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 12421 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 12481 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 12541 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 12601 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 12661 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 12721 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 12781 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTG //