LOCUS New_DNA 13429 bp ds-DNA circular 06-FEB-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from 91854 pORF-VPS72_ORS02546 652 to 1779 COMMENT Backbone from 90946 PB-tet-PH-lox66FRT2 6253 to 4570 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 5688..5698 /mol_type="other DNA" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /db_xref="taxon:32630" /organism="synthetic construct" /clone_lib="HIP_Gateway221_closed /focus /note=Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1205..1459 /label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 source 4581..4592 /mol_type="other DNA" /clone="IMAGE:100003590; FLH168003.01X; RZPDo839A1192D" /db_xref="taxon:32630" /organism="synthetic construct" /clone_lib="HIP_Gateway221_closed /focus /note=Vector: pDONR221; derived from parent clone IMAGE:3451917 (accession BC001301.1)" /label=Vector: pDONR221; derived from parent clone IMAGE:(1) /ApEinfo_label=Vector: pDONR221; derived from parent clone IMAGE: /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3331..3678 /label=polyA /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(463..531) /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5688..5688 /note="linker at the 3' end of the ORF" /label=linker at the 3' end of the ORF /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1460..1711 /label=mouse PGK promoter(1) /ApEinfo_label=mouse PGK promoter /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 707..941 /label=PB 3'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4593..5687 /label=VPS72(BC003151) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 6422..6490 /label=LacZ alpha(1) /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4446..4551 /label=mCMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4581..4587 /note="attL1 site" /label=attL1 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5745..6268 /label=IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(11052..11085) /label=CoreFRT /ApEinfo_fwdcolor=#8080c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3685..3926 /label=chicken 5'-HS4 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5689..5698 /note="attL2 site" /label=attL2 site /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4588..4592 /note="linker at the 5' end of the ORF that includes ACC for minimal Kozak consensus" /label=linker at the 5' end of the ORF that includes ACC /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10972..11005 /label=lox66 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4160..4437 /label=8xTetO2 element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(5699..5713) /label=AttR2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 602..619 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11038..11085 /label=FRT /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 618..645 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6270..10212 /label=b-geo /ApEinfo_fwdcolor=#800000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1732..2328 /label=Pac /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6333..9358 /label=LacZ /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10213..10519 /label=pA /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 11038..11050 /label=FRTunit /ApEinfo_fwdcolor=#80ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10637..10907 /label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11236..11255) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(135..441) /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(11273..11293) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(3..458) /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(11661..12343) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2335..3330 /label=DeltaTK /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding complement(11299..11321) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(12441..13100) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4563..4570 /label=AttR1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10908..10949 /label=PB 5'LTR(1) /ApEinfo_label=PB 5'LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG 661 TATTCACGAC AGCAGGCTGA ATAATAAAAA AATTAGAAAC TATTATttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaAA CATTCTCTCT 961 TTTACAAAAA TAAACTTATT TTGTACTTTA AAAACAGTCA TGTTGTATTA TAAAATAAGT 1021 AATTAGCTTA ACCTATACAT AATAGAAACA AATTATACTT ATTAGTCAGT CAGAAACAAC 1081 TTTGGCACAT ATCAATATTA TGCTCTCGTT ACTGGCGAAA GGGGGATGTG CTGCAAGGCG 1141 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA 1201 ATTCtaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 1261 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 1321 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 1381 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 1441 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 1501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 1561 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 1621 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 1681 ctcctcttcc tcatctccgg gcctttcgac cGATCATCAA GCTTGATCCT Catgaccgag 1741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccAgggccgt acgcaccctc 1801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgaTccgga ccgccacatc 1861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 1921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 1981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 2041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 2101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 2161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 2221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 2281 Gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccATGCCC 2341 ACGCTACTGC GGGTTTATAT AGACGGTCCC CACGGGATGG GGAAAACCAC CACCACGCAA 2401 CTGCTGGTGG CCCTGGGTTC GCGCGACGAT ATCGTCTACG TACCCGAGCC GATGACTTAC 2461 TGGCGGGTGc TGGGGGCTTC CGAGACAATC GCGAACATCT ACACCACACA ACACCGCCTC 2521 GACCAGGGTG AGATATCGGC CGGGGACGCG GCGGTGGTAA TGACAAGCGC CCAGATAACA 2581 ATGGGCATGC CTTATGCCGT GACCGACGCC GTTCTGGCTC CTCATATCGG GGGGGAGGCT 2641 GGGAGCTCAC ATGCCCCGCC CCCGGCCCTC ACCCTCATCT TCGACCGCCA TCCCATCGCC 2701 GCCCTCCTGT GCTACCCGGC CGCGCGgTAC CTTATGGGCA GCATGACCCC CCAGGCCGTG 2761 CTGGCGTTCG TGGCCCTCAT CCCGCCGACC TTGCCCGGCA CcAACATCGT GcTTGGGGCC 2821 CTTCCGGAGG ACAGACACAT CGACCGCCTG GCCAAACGCC AGCGCCCCGG CGAGCGGCTg 2881 GACCTGGCTA TGCTGGCtGC GATTCGCCGC GTTTAcGGGC TaCTTGCCAA TACGGTGCGG 2941 TATCTGCAGt GCGGCGGGTC GTGGCGGGAG GAcTGGGGAC AGCTTTCGGG GaCGGCCGTG 3001 CCGCCCCAGG GTGCCGAGCC CCAGAGCAAC GCGGGCCCAC GACCCCATAT CGGGGACACG 3061 TTATTTACCC TGTTTCGGGC CCCCGAGTTG CTGGCCCCCA ACGGCGACCT GTATAACGTG 3121 TTTGCCTGGG CcTTGGACGT CTTGGCCAAA CGCCTCCGTT CCATGCACGT CTTTATCCTG 3181 GATTACGACC AATCGCCCGC CGGCTGCCGG GACGCCCTGC TGCAACTTAC CTCCGGGATG 3241 GTCCAGACCC ACGTCACCAC CCCCGGCTCC ATACCGACGA TATGCGACCT GGCGCGCACG 3301 TTTGCCCGGG AGATGGGGGA GGCTAACTGA gctctagctt atcgactcgc cgatagtgga 3361 aaccgacgcc ccagcactcg tccgagggca aaggaatagt cgagaaattg atgatctatt 3421 aaacaataaa gatgtccaca tggaagtttt tcctgtcata ctttgttaag aagggtgaga 3481 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga 3541 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca 3601 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca 3661 ctcatgatct atagatccTc tagaGAGCTC ACGGGGACAG CCCCCCCCCA AAGCCCCCAG 3721 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 3781 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 3841 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 3901 CACCTGGGGG ATACGGGGAA AAAGCTgtcg aCCTGCAGGC ATGCAAGCTT GGCGTAATCA 3961 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4021 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4081 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGTA ACTCGAGGGA 4141 TCCAAGCTgg CCgcACGTCT CCCTATCAGT GATAGAGAAG TCGACACGTC TCGAGCTCCC 4201 TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT CAGTGATAGA GAAGTCGACA 4261 CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA GAACGTCTCC CTATCAGTGA 4321 TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG AGAAGGTACG TCTAGAACGT 4381 CTCCCTATCA GTGATAGAGA AGTCGACACG TCTCGAGCTC CCTATCAGTG ATAGAGAAGG 4441 TACCCCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC 4501 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GTGGCGGCCA 4561 TCACAAGTTT gtacaaaaaa gcaggctcca ccatgagttt ggctgggggc cgggcacccc 4621 ggaagaccgc tgggaaccgg ctttctgggc ttttggaggc agaggaggaa gatgagttct 4681 accagacgac ttatgggggt ttcacagagg aatccggaga tgatgagtat caaggggacc 4741 agtcagacac agaggacgaa gtggactctg actttgacat tgatgaaggg gatgaaccat 4801 ccagtgatgg agaagcagaa gagccaagaa ggaagcgccg agtagtcacc aaggcctata 4861 aggaacctct caagagctta aggcctcgaa aggtcaacac cccggctggt agctctcaga 4921 aggcgcgaga agagaaggca ctactgccat tagaactaca agatgacggc tctgacagtc 4981 ggaagtctat gcgtcagtct acagctgagc atacacgaca aacgttcctt cgggtacagg 5041 agaggcaggg ccagtcaaga cggcgaaagg ggccccactg tgagcggcca ctaacccagg 5101 aggaactgct ccgggaggcc aagatcacag aagagcttaa tttacggtca ctggagacat 5161 atgagcggct cgaggctgat aaaaagaagc aggttcataa gaagcggaag tgccccgggc 5221 ccataatcac ctatcattca gtgacagtgc cacttgttgg ggagccaggc cccaaggaag 5281 agaacgttga catagaagga cttgatcctg ctccctcggt gtctgcattg actcctcatg 5341 ctgggactgg acccgtcaac ccccctgctc gctgctcacg taccttcatc acttttagtg 5401 atgatgcaac tttcgaggaa tggttccccc aagggcggcc cccaaaagtc cctgttcgtg 5461 aggtctgtcc agtgacccat cgtccagccc tataccggga ccctgttaca gacataccct 5521 atgccactgc tcgagccttc aagatcattc gtgaggctta caagaagtac attactgccc 5581 atggactgcc gcccactgcc tcagccctgg gccccggccc gccacctcct gagcccctcc 5641 ctggctctgg gccccgagcc ttgcgccaga aaattgtcat taaataggac ccagctttCT 5701 TGTACAAAGT GGTGATggcc GCTCTAGCTA GTCGATAAGC TAATTCCGCC CCCCCCCCCC 5761 CCCCCCCCTC ACCcTCCCCC CCCCctaacg ttactggccg aagccgcttg gaataaggcc 5821 ggtgagcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg 5881 cccgaaacct ggccctgtct tcttgacgac gattcctagg ggtctttccc ctctcgccaa 5941 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 6001 acaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacatggat 6061 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc 6121 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg 6181 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttggtt 6241 tgaaaaacac gatgataata tggccacaac catggaacag ggggatcagc ttaccatggc 6301 caagatccct cctaagaaga agcgcaaagt cgaggatccc gtcgttttac aacgtcgtga 6361 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 6421 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 6481 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga 6541 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta 6601 cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc cgtttgttcc 6661 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca 6721 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa 6781 cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc 6841 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga gtgacggcag 6901 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct 6961 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt 7021 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg 7081 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg 7141 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt 7201 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact 7261 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt 7321 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa 7381 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga 7441 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca 7501 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat 7561 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc 7621 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat 7681 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat 7741 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc 7801 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt 7861 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct 7921 ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc 7981 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct 8041 gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac 8101 gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc 8161 agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac 8221 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat 8281 ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca 8341 aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg 8401 gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag 8461 cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca 8521 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg 8581 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca 8641 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt 8701 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca 8761 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct 8821 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac 8881 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga 8941 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt 9001 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt 9061 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa 9121 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt 9181 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct 9241 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg 9301 cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg gtcgctacca 9361 ttaccagttg gtctggtgtc aggggatccc ccgggctgca gccaatatgg gatcggccat 9421 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 9481 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 9541 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 9601 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 9661 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 9721 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 9781 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 9841 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 9901 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 9961 tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 10021 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 10081 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 10141 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 10201 gttcttctga ggggatcaat tctctagggc tcgctgatca gcctcgactg tgccttctag 10261 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 10321 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 10381 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 10441 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 10501 ctcgatcctc tagagtcgaG GGCTGCAGGA ATTCGATATC AAGCTCTAGC CAACAAGCTC 10561 GTCATCGCTT TGCAGAAGAG CAGAGAGGAT ATGCTCATCG TCTAAAGAAC TACCCATTTT 10621 ATTATATATT AGTCACgata tctataacaa gaaaatatat atataataag ttatcacgta 10681 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa 10741 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta 10801 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca 10861 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaATG CATgcgtcaa 10921 ttttacgcag actatctttc tagggttaaA tttGGGCGGT CGAGGGACCT Aataacttcg 10981 tataGCATAC Attatacgaa cggtaATTAA GGGTTCCGGA TCCCCATCAA GCTAATTgaa 11041 gttcctattc Cgaagttcct attcTCTAGA AAgtatagga acttcCAGCT GGAAAtGATT 11101 TGCGCTTTAC TCGACCTAAA CTTTAAACAC GTCATAGAAT CTTCGTTTGA CAAAAACCAC 11161 ATTGTGGCCA AGCTGTGTGA CGCGACGCGC GCTAAAGAAT GGCAAACCAA GTCGCGCGAG 11221 GTACCCAGCT TTTGTTCCCT TTAGTGAGGG TTAATTCCGA GCTTGGCGTA ATCATGGTCA 11281 TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA 11341 AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG 11401 CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC 11461 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC 11521 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA 11581 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 11641 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 11701 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 11761 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 11821 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 11881 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 11941 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 12001 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 12061 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG 12121 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 12181 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 12241 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 12301 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC 12361 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG 12421 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT 12481 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG 12541 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA 12601 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT 12661 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA 12721 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG 12781 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC 12841 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG 12901 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA 12961 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT 13021 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC 13081 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC 13141 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA 13201 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA 13261 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT 13321 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 13381 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTG //